Setting up. The standard inverted microscope is upgraded to a high quality live imaging station. Credit: Linda Koffmar
A recent study from Uppsala University shows how smartphones can be used to make movies of living cells, without the need for expensive equipment. The study is published in the open access journal PLOS ONE, making it possible for laboratories around the world to do the same thing.
Live imaging of cells is a very powerful tool for the study of cells, to learn about how cells respond to different treatments such as drugs or toxins. However, microscopes and equipment for live imaging are often very expensive.
In the present study, old standard inverted microscopes that are very abundant at Universities and hospitals were upgraded to high quality live imaging stations using a few 3D-printed parts, off-the-shelf electronics, and a smartphone. It was shown that the resultant upgraded systems provided excellent cell culture conditions and enabled high-resolution imaging of living cells.
ATLIS: an affordable system for time-lapse imaging and incubation of cells. (a) The basic version of ATLIS was assembled by the combination of four modules. The imaging module, composed of a smartphone, a 3D-printed holder, and a motorized shutter, was designed to capture images of cells at a fixed interval minimizing their exposure to light. The heating unit was created to warm up the air introduced into a 3D-printed onstage incubator where cells are kept. Finally, the control unit was programmed to maintain a constant temperature inside the incubator by adjusting the power supplied to the heating unit. (b) A photograph of the ATLIS mounted on a Nikon TMS microscope fitted with a Samsung Galaxy S I9000 smartphone. (1) Nikon TMS microscope; (2) Samsung Galaxy S I9000 smartphone; (3) smartphone holder; (4) onstage incubator; (5) heating unit; (6) power supply; (7) control unit; (8) shutter; (9) temperature sensor; (10) light source.
“What we have done in this project isn’t rocket science, but it shows you how 3D-printing will transform the way scientists work around the world. 3D-printing has the potential to give researchers with limited funding access to research methods that were previously too expensive,” says Johan Kreuger, senior lecturer at the Department of Medical Cell Biology at Uppsala University.
“The technology presented here can readily be adapted and modified according to the specific need of researchers, at a low cost. Indeed, in the future, it will be much more common that scientists create and modify their own research equipment, and this should greatly propel technology development,” says Johan Kreuger.
http://www.uu.se/en/media/news/article/?id=7940&area=2,4,10,16,24&typ=artikel&lang=en http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0167583
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