CRISPR/Cas9 tagged posts

An international team led by the Case Western Reserve University School of Medicine has discovered novel properties of the protein Gasdermin B that promotes repair of cells lining the gastrointestinal tract in people with chronic inflammatory disorders like Crohn’s disease and ulcerative colitis.

The new findings, recently published in the journal, Cell, are significant because the impact of Gasdermin B (GSDMB) on healing epithelium — a type of body tissue that lines the organs that have direct contact with the external environment — will play a key role in research on wound formation and designing novel therapeutics to enhance wound repair, said Theresa Pizarro, lead study author and the Louis Pillemer Professor of Experimental Pathology at the School of Medicine...

Knocking out a protein known to stifle T cell activation on CAR T cells using the CRISPR/Cas9 technology enhanced the engineered T cells’ ability to eliminate blood cancers, according to new preclinical data from researchers in the Perelman School of Medicine at the University of Pennsylvania and Penn’s Abramson Cancer Center.

The findings will be presented as an oral presentation at the 62nd American Society of Hematology Annual Meeting & Exposition on Dec. 7 (abstract 554).

The team knocked out the CD5 gene — which encodes for the CD5 protein on the surface of T cells and can inhibit their activation — on CAR T cells using CRISPR-Cas9 and infused them back into mice with T- and B-cell leukemia or lymphoma...

Study in mice shows promise for treating genetic conditions during early stages of development. Researchers at Carnegie Mellon University and Yale University have for the first time used a gene editing technique to successfully cure a genetic condition in a mouse model. Their findings, published in Nature Communications, present a promising new avenue for research into treating genetic conditions during fetal development.

An estimated 8 million children are born each year with severe genetic disorders or birth defects. Genetic conditions can often be detected during pregnancy using amniocentesis, but there are no treatment options to correct these genetic conditions before birth. “Early in embryonic development, there are a lot of stem cells dividing at a rapid pace...

A magenta-stained cell shows how the molecule STING (green) is positioned next to the bacteria Listeria (blue) to start an immune response. Credit: Washington State University

Discovery opens door to efficient research model. Washington State University researchers have seen how both humans and fruit flies deploy a protein that plays a critical role in their immune responses to invading bacteria. The discovery gives scientists a model organism with which to explore ways to boost the human immune system and create infection-fighting medicines.

Naturally, there are enormous differences between humans and fruit flies, whose common ancestor goes back 800 million years...