CRISPR/Cas9 tagged posts

Histological sections of liver from control mice treated with saline (left) and the CRISPR/Cas9 epigenetic repression system in which cholesterol levels were lowered (right) show generally normal and healthy tissue. Credit: Charles Gersbach, Duke University

Technique allowed researchers to reduce blood cholesterol levels in adult mice for six months following a single treatment. Biomedical engineers at Duke University have used a CRISPR/Cas9 genetic engineering technique to turn off a gene that regulates cholesterol levels in adult mice, leading to reduced blood cholesterol levels and gene repression lasting for six months after a single treatment.

This marks the first time researchers have delivered CRISPR/Cas9 repressors for targeted therapeutic gene silencing in adult animal models...

Charles Gersbach, the Rooney Family Associate Professor of Biomedical Engineering at Duke University. Credit: Duke University

Researchers have developed a method to swiftly the non-coding DNA of the human genome for links to diseases that are driven by changes in gene regulation. The technique could revolutionize modern medicine’s understanding of the genetically inherited risks of developing heart disease, diabetes, cancer, neurological disorders and others, and lead to new treatments.

“Identifying single mutations that cause rare, devastating diseases like muscular dystrophy has become relatively straightforward,” said Charles Gersbach, the Rooney Family Associate Professor of Biomedical Engineering at Duke University...

Chimeric antigen receptors (CARs): (a) anatomy and evolution of CAR design, and (b) CAR T cells recognize specific antigens on cancer cell surface and trigger T-cell–mediated killing.

Researchers from Memorial Sloan Kettering Cancer Center have harnessed the power of CRISPR/Cas9 to create more-potent chimeric antigen receptor (CAR) T cells that enhance tumor rejection in mice. The unexpected findings uncover facets of CAR immunobiology and underscore the potential of CRISPR/Cas9 genome editing to advance immunotherapies for cancer.

CRISPR is a genome-editing tool that enables scientists to cut and manipulate a cell’s DNA with high precision. In the Nature paper, MSK investigators show that CRISPR technology can deliver the CAR gene to a very specific location in the genome of the T cell...

A visual description of the CRISPR/Cas9 research explored by UNT researchers using TACC supercomputers. Simulations found that Cas9 produces staggered, “sticky” ends, which may make them easier to manipulate for future use. Credit: Jin Liu and Zhicheng Zuo, UNT

The first all-atom molecular dynamics simulations of Cas9-catalyzed DNA cleavage in action has been performed. The simulations shed light on the process of Cas9 genome editing and helped resolve controversies about specific aspects of the cutting. Originally found as part of the immune system of the Streptococcus pyogenes bacteria, CRISPR associated protein 9 (CAS9), in its native state, recognizes foreign DNA sequences and disables them...