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    gene editing tagged posts

    Proofreading the book of life: Gene Editing made Safer

    April 24, 2019, Categories  Biology/Biotechnology, Health/Medical

    The graphic illustrates the technique described in the new study. A version of the Cas9 protein used in CRISPR gene editing has been mutated. While this protein, seen in blue, retains its proper functioning, it remains “immunosilent”–hidden from predation as a foreign entity by the immune system’s T cells (seen in brown).
    Credit: Graphic by Jason Drees

    Scientists describe a method of rendering the gene editing tool CRISPR-Cas9 ‘immunosilent,’ potentially allowing the editing and repair of genes to be accomplished reliably and stealthily...

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    DNA Nanoscale Vehicle 1st time used to deliver CRISPR-Cas9 Gene-Editing tool into cells in both cell culture, animal model

    August 28, 2015, Categories  Biology/Biotechnology, Technology/Electronics

    When the nanoclew comes into contact with a cell, the cell absorbs the nanoclew completely -- swallowing it and wrapping it in a protective sheath called an endosome. But the nanoclews are coated with a positively charged polymer that breaks down the endosome, setting the nanoclew free inside the cell. The CRISPR-Cas9 complexes can then free themselves from the nanoclew to make their way to the nucleus. Credit: North Carolina State University

    When the nanoclew comes into contact with a cell, the cell absorbs the nanoclew completely — swallowing it and wrapping it in a protective sheath called an endosome. But the nanoclews are coated with a positively charged polymer that breaks down the endosome, setting the nanoclew free inside the cell. The CRISPR-Cas9 complexes can then free themselves from the nanoclew to make their way to the nucleus. Credit: North Carolina State University

    CRISPR-Cas system, found in bacteria and archaea, protects bacteria from invaders such as viruses. CRISPR RNA has been used in gene-editing to ID a targeted portion of relevant DNA, and Cas protein cleaves it. But for Cas9 to do its work, it must first find its way into the cell...

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