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Gravitational Biology: Real Time Imaging and Transcriptome Analysis of Fish aboard Space Station

a-d) Whole-body imaging of the osterix-DsRed transgenic line. The left-side images show the same ground control at day 1; and the right-side images, the same flight medaka at day 1. Arrows point to the head and fin region. All images show ventral views. Montage images were made from 6 captured optical images, divided by dotted lines (a,b). The white region shows an osterix-DsRed fluorescent signal. Embedded views show the enlarged head region (c,d). (e) The fluorescent intensity from day 1 to 7 of observation day constantly increased in the flight group. (f-h) The representative visualizing data for osterix-DsRed/TRAP-GFP in the flight group. All images show ventral views in the head region. (i-l) The merged images were captured by 3D views for osterix-DsRed and TRAP-GFP in the pharyngeal bone region of the double transgenic line. The pharyngeal bone region in the ground control (i) or the flight (k) group at day 4. The image for TRAP-GFP in the pharyngeal bone region of "i" (j) or "k" (l). lp, lower pharyngeal bone; c, cleithrum. GFP signals identify osteoclasts (OC). Credit: Tokyo Institute of Technology

a-d) Whole-body imaging of the osterix-DsRed transgenic line. The left-side images show the same ground control at day 1; and the right-side images, the same flight medaka at day 1. Arrows point to the head and fin region. All images show ventral views. Montage images were made from 6 captured optical images, divided by dotted lines (a,b). The white region shows an osterix-DsRed fluorescent signal. Embedded views show the enlarged head region (c,d). (e) The fluorescent intensity from day 1 to 7 of observation day constantly increased in the flight group. (f-h) The representative visualizing data for osterix-DsRed/TRAP-GFP in the flight group. All images show ventral views in the head region...

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