Serum leptin and adipocyte leptin mRNA levels decrease with dietary iron overload. (A) Iron levels measured by ferrozine-based colorimetric assay in mice fed low normal– and high-iron diet (n = 4 mice/group). (B) Tfrc mRNA levels quantified by qPCR and normalized to cyclophilin A in adipose tissue from mice fed different levels of dietary iron (n = 6 mice/group). (C) Serum leptin levels from the mice (n = 6 mice/group). (D) Intracellular leptin levels by Western blotting. Data are representative of 3 experiments with n = 4 per replicate. (E) Quantification of leptin protein levels from D normalized to tubulin (n = 4 mice/group). (F) Leptin mRNA levels quantified by qPCR and normalized to cyclophilin A in adipose tissue from mice fed different dietary iron (n = 6 mice/group)...
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