Interfering with Tau N-terminal-dependent vesicle-binding reverts Tau-induced presynaptic deficits in fly neurons. Drosophila larvae used in (a–c) express UAS-TauΔN (R406W, V337M or P301L) under the D42-Gal4 motor neuron driver. (a) FRAP measurements of vesicle mobility within synaptic boutons. Fluoresence recovery (% of initial fluorescence) was plotted over time and fit with double-exponential curves. n=22 (R406W), 20 (ΔN_R406W, ΔN_V337M), 24 (ΔN_P301L) or 25 (Control) boutons (3–5 boutons per animal). (b) Synapto-pHluorin responses to stimulation at 10 Hz with the presence of bafilomycin. Fluorescence change ΔF at ratio to maximal ΔF (NH4Cl dequenching) was plotted over time during the stimulation. Two-way ANOVA, n=7 (R406W, ΔN_R406W, ΔN_V337M, ΔN_P301L),9 (Control) NMJs (animals)...
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