lifespan extension tagged posts

Gcn4 strongly represses translation in the long-lived RPKO strains and stresses. Quantification of global translation by Click-iT HPG for a different single and double KO strains, b glucose-starved (CR) and rapamycin-treated (RAPA) yeast cells. GCN4 deletion restores translation to the level of the wild-type strain in RPKO strains and also leads to increased translation in stressed cells. The significance of the two-tailed t-test between any given deletion strain and the wild-type strain is depicted above the respective bar. Mean values of the relative translation change between the GCN4 deletion strain and the respective parental strain are shown in parentheses. Error bars represent s.d. across three different biological replicates except glucose starvation where n = 2...

With disease, when you decrease autophagy (a garbage disposal-like process where cells “eat” debris they produce) the disease process is exacerbated and when you increase it you get the opposite effect. Aggregation of polyglutamine expansion protein is a hallmark of Huntington’s disease and other neurodegenerative diseases. The picture shows that there are more aggregates of green fluorescence protein-labelled polyglutamine expansion protein in autophagy deficient worms (bottom) compared to normal worms (top).