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Effects of pNaKtide on adipocyte phenotype, senescence, and apoptosis in C57Bl6 aging mice. (A) H&E staining in visceral adipose tissue. Images taken with 40X objective lens; scale bar represents 100 µm. Arrows mark “crown like structures” indicative of inflammation. (B) Quantitative analysis of adipocytes area in visceral adipose tissue. (C) Representative images of TUNEL assay with quantification in C57B16 aging mice. Images taken with 40X objective lens; scale bar represents 25 µm. (D–F) qRT-PCR analysis of ApoJ, p21 and PPARγ in C57Bl6 aging mice with GAPDH as a loading control. Y, young; Y + P, young + pNaKtide; OB, old baseline; O, old; O + P, old + pNaKtide; O + WD, old + western diet; O + WD + P, old + western diet + pNaKtide. N = 8/group, *p < 0.05, **p < 0...
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