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    Nanosecond pulsed electric fields (nsPEFs) tagged posts

    Nanosecond Pulsed Electric fields activate Immune cells

    August 9, 2019, Categories  Biology/Biotechnology, Health/Medical

    figure3
    Analysis of extracellular DNA by agarose gel electrophoresis. (A) Schematic representation of MNase treatment for liberation of extruded DNA from cells. MNase digests extracellular chromosomal DNA at its linker regions among nucleosomes, leading to the liberation of extracellular chromosomal DNA from their originating cells. Liberated DNA was separated from cells by brief centrifugation and in turn subjected to either DNA purification followed by agarose gel electrophoresis (B) or direct fluorometric measurement with a SYTOX Green dye (Fig. 4). (B) Analysis of MNase-treated extracellular DNA by agarose gel electrophoresis. Differentiated (D) and undifferentiated cells (U) were exposed to the indicated shot numbers of 20 kV/cm nsPEFs...
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