protein map for sepsis tagged posts

Construction of a tissue-specific protein abundance atlas. (a) Vascularized organs, plasma and cells adjacent to the blood plasma were collected from healthy Balb-C mice. The collected organs and cells were washed in PBS, homogenized, the proteins digested with trypsin and analysed by shotgun LC-MS/MS. (b) The scaled spectral counts from the LC-MS/MS analysis of the individual organs and cells were correlated using Pearson’s r correlation coefficient, indicated by the numbers in the heat map. (c) Heat map of the scaled spectral counts of the different cell and organs. The grey lines in the heat map shows percentage of signal associated with a given organ or cell type. Total number of identified proteins per organ, blood vessel and cells is shown in brackets below the heat map.