Timeless tagged posts

Redox-sensitive alteration of replisome architecture safeguards genome integrity. Science, 2017; 358 (6364): 797 DOI: 10.1126/science.aao3172

New DNA is generated in human cells from nucleotides produced by an enzyme RNR. Until now, we have not fully understood how exactly the RNR rhythm and the presence of right amount of nucleotides are aligned with the pace of DNA replication. Now, researchers from the Faculty of Health and Medical Sciences at the University of Copenhagen have mapped the flow and regulation of nucleotides. The flow follows the same rhythm as replication of DNA does – and when it does not, the cells regulate the process to align the two.

“We can see that these processes follow the same periodic rhythm...

Highlights •Timeless interacts with PARP-1 independent of poly(ADP-ribosyl)ation •Crystal structures of Timeless PAB in free form and in complex with PARP-1 •Specific recognition of PARP-1 by Timeless does not affect its enzymatic activity •PARP-1 is required for Timeless recruitment to DSB sites to promote HR repair

A new molecular mechanism for DNA repair involving an unexpected interaction between PARP-1 protein and sleep proteins in humans has been found. While it is a well-known fact that sleep is important to the body’s recovery and healing, knowledge of the molecular mechanisms that make this possible is incomplete...