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    Surprise COVID Discovery Helps Explain How Coronaviruses Jump Species

    August 15, 2023, Categories  Biology/Biotechnology, Health/Medical

    DNA-tethering and fusion of SARS-CoV-2 pseudoviruses and virus-like particles (VLP). The experiment design is schematized in panel (a), where DNA-functionalized viral particles are added to a microfluidic flow cell and allowed to bind to protein-free liposomes functionalized with complementary DNA. After unbound particles are washed away, fusion is initiated by addition of a soluble protease and monitored via lipid mixing, detected as fluorescence dequenching of Texas Red dye in the VLP or pseudoviral envelope. Representative images of a 10.1 × 9.6 μm sub-micrograph before and after lipid mixing are shown in panel (b) with a fusing particle outlined in magenta. The corresponding fluorescence intensity trace is plotted in panel (c).

    Unexpected new insights into how COVID-19 infects ce...

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