TDP1 promotes mitochondrial gene transcription in human cells.
A 5-yr study has found the mechanism responsible for repairing damage to mitochondrial DNA. This discovery could pave the way for new treatments for cancer and neurodegenerative diseases. This research may also have important implications for clinical advances in so called ‘three-parent baby’ mitochondrial donation. They also show how the enzyme TDP1- already known to have a role in repairing damaged DNA in the cell’s nucleus – is also responsible for repairing damage to mitochondrial DNA (mtDNA).
During the process of energy production and making proteins, a large amount of rogue reactive oxygen species are produced which constantly attack the DNA in the mitochondria. These attacks break their DNA, however the new findings show mitochondria have their very own repair toolkits which are constantly active to maintain their own DNA integrity. Professor Sherif El-Khamisy, a Wellcome Trust Investigator and Chair of Molecular Medicine at the University of Sheffield, said: “Each mitochondria repair toolkit has unique components — enzymes – which can cut, hammer and seal the breaks. The presence of these enzymes is important for energy production.
“Defects in repairing DNA breaks in the mitochondria affect vital organs that rely heavily on energy such as the brain. It also has implications on mitochondria replacement therapies recently approved in the UK and known as ‘three parent babies’.” Although much research has focused on how free radicals damage the DNA in the cell’s nucleus, their effect on mitochondrial DNA is less well understood despite this damage to mtDNA being responsible for many different types of disease such as neurological disorders.
Having healthy mitochondria is also essential for tissue regeneration, making it particularly important for successful organ transplants. The team further identified a mechanism through which mtDNA can be damaged and then fixed, via a protein called TOP1, responsible for untangling coils of mtDNA. When the long strands become tangled, TOP1 breaks and quickly repairs the strands to unravel the knots. If free radicals are also attacking the mitochondrial DNA, then TOP1 proteins can become trapped on the mitochondrial DNA strands, making repair even more difficult.
Professor El-Khamisy believes the findings could pave the way for the development of new therapies for mitochondrial disease that boost their DNA repair capacity, or for cancer treatments which could use TDP1 inhibitors to prevent mtDNA repair selectively in cancer cells. “Cancer relies on cells dividing very quickly. That means they need a lot of energy, so will have really healthy mitochondria,” said Professor El-Khamisy.
“If we can find a way to selectively damage the mitochondria in the cancer cells, by preventing or slowing its repair mechanism, this could be really promising.” The findings could also be important for new clinical advances such as the decision by the Human Fertilisation and Embryology Authority (HFEA) to allow ‘mitochondrial donation’ – also known as ‘three-parent babies’ – where mtDNA from a female donor is introduced to an embryo to correct mitochondrial defects.
“This research suggests that clinicians should assess the function of TDP1 and mitochondrial TOP1 before mitochondrial donation takes place, to ensure the success of this procedure,” added Professor El-Khamisy. “Even if the new embryo has healthy mitochondrial DNA from the donor, it could still have defective TDP1 or mitochondrial TOP1 from the recipient, since they are both produced by the DNA in the cell’s nucleus, so mitochondrial DNA damage could still take place over time, and cause disease.” http://www.sheffield.ac.uk/news/nr/sheffield-research-new-cancer-treatments-1.698006 http://advances.sciencemag.org/content/3/4/e1602506/tab-figures-data
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