This image shows the shapes made of living tissue made by the researchers. By patterning mechanically active mouse or human cells to thin layers of extracellular fibers, the researchers could create bowls, coils, and ripple shapes. Credit: Alex Hughes
Many of the complex folded shapes that form mammalian tissues can be recreated with very simple instructions, UC San Francisco bioengineers report December 28 in the journal Developmental Cell. By patterning mechanically active mouse or human cells to thin layers of extracellular matrix, ECM fibers, the researchers could create bowls, coils, and ripples out of living tissue. The cells collaborated mechanically through a web of these fibers to fold themselves up in predictable ways, mimicking natural developmental processes.
“Development is starting to become a canvas for engineering, and by breaking the complexity of development down into simpler engineering principles, scientists are beginning to better understand, and ultimately control, the fundamental biology,” says senior author Zev Gartner, part of the Center for Cellular Construction at the University of California, San Francisco. “In this case, the intrinsic ability of mechanically active cells to promote changes in tissue shape is a fantastic chassis for building complex and functional synthetic tissues.
Labs already use 3D printing or micro-molding to create 3D shapes for tissue engineering, but the final product often misses key structural features of tissues that grow according developmental programs. The Gartner lab’s approach uses a precision 3D cell-patterning technology called DNA-programmed assembly of cells (DPAC) to set up an initial spatial template of a tissue that then folds itself into complex shapes in ways that replicate how tissues assemble themselves hierarchically during development,
“We’re beginning to see that it’s possible to break down natural developmental processes into engineering principles that we can then repurpose to build and understand tissues,” says Alex Hughes, a postdoctoral fellow at UCSF. “It’s a totally new angle in tissue engineering…. It was astonishing to me about how well this idea worked and how simply the cells behave,” Gartner says. “This idea showed us that when we reveal robust developmental design principles, what we can do with them from an engineering perspective is only limited by our imagination. Alex was able to make living constructs that shape-shifted in ways that were very close to what our simple models predicted.”
Gartner and his team are now curious to learn whether they can stitch the developmental program that control tissue folding together with others that control tissue patterning. They also hope to begin to understand how cells differentiate in response to the mechanical changes that occur during tissue folding in vivo, taking inspiration from specific stages of embryo development.
https://www.ucsf.edu/news/2017/12/409516/engineers-hack-cell-biology-create-3d-shapes-living-tissue
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