Category Biology/Biotechnology

Scanning electron micrograph of cultured human neuron from induced pluripotent stem cell. Credit: Mark Ellisman and Thomas Deerinck, National Center for Microscopy and Imaging Research, UC San Diego

Researchers at UCSD School of Medicine report that they have successfully created spinal cord neural stem cells (NSCs) from human pluripotent stem cells (hPSCs) that differentiate into a diverse population of cells capable of dispersing throughout the spinal cord and can be maintained for long periods of time...

An illustration of how CAS-12A works with DNA. Illustration by Jenna Luecke, University of Texas at Austin

Scientists have found conclusive evidence that Cas9, the most popular enzyme currently used in CRISPR gene editing, is less effective and precise than one of the lesser-used CRISPR proteins, Cas12a. Because Cas9 is more likely to edit the wrong part of a plant’s or animal’s genome, disrupting healthy functions, the scientists make the case that switching to Cas12a would lead to safer and more effective gene editing.

“The overall goal is to find the best enzyme that nature gave us and then make it better still, rather than taking the first one that was discovered through historical accident,” said Ilya Finkelstein, an assistant professor of molecular biosciences and a co-author of the ...

Highlights
• Loss of IRF4 in BAT causes decreased exercise capacity and a selective myopathy
• IRF4 represses myogenic genes in BAT, including the myokine myostatin
• Neutralizing serum myostatin rescues the ability of BATI4KO mice to exercise normally
•Warming reduces IRF4 in BAT, causing myopathy that is reversed by removal of BAT

Researchers open the door to potential new therapies for certain metabolic and muscular diseases. Now, endocrinologists at Beth Israel Deaconess Medical Center (BIDMC) have shown for the first time that brown adipose tissue (BAT), or brown fat, can exert control over skeletal muscle function. Alterations to the brown adipose tissue in mice resulted in a significant and consistent reduction in exercise performance...

This figure shows cross sectional MRI images are displayed with pixels colour-coded for fat content. In this individual, the high liver fat at baseline (30.4 percent) decreased to 1.3 percent after weight loss. The lower panels, at the level of the pancreas (outlined in white), show the decrease in pancreas fat (8.9 to 7.5 percent) associated with the restoration of first phase and maximal insulin secretion.
Credit: Taylor et al.

A clinical trial recently showed that nearly half of individuals with type 2 diabetes achieved remission to a non-diabetic state after a weight-loss intervention delivered within 6 years of diagnosis...