Recent Comments

    Category Health/Medical

    Distinct Wiring mode found in Chandelier Cells

    June 10, 2017, Categories  Health/Medical, Biology/Biotechnology

    AIS but not off-target varicosities predominantly contain presynaptic markers. A, Immunohistochemical analysis of VGAT localization in ChC varicosities in Nkx2.1-CreER;LSL-tGFP mice. Confocal images of a single optical section showing varicosities (green), VGAT (red), and AISs visualized by AnkG immunostaining (blue) in a P16 brain. White and yellow arrowheads indicate AIS and off-target varicosities, respectively. Filled and empty arrowheads show VGAT+ and VGAT- varicosities in ChC axons, respectively. Scale bar, 5 μm. B, The average percentage of VGAT-containing AIS and off-target varicosities in P16 ChCs. The percentage of VGAT-containing AIS varicosities is significantly higher than that of VGAT-containing off-target varicosities (n = 7 ChCs for each; ≥3 brains per condition; Student’s t test: **p < 0.001)h. Data are presented as mean ± SEM. C, Localization of Syp-YFP puncta in varicosities of transplanted ChCs. Confocal images of a single optical section showing AIS visualized by AnkG immunostaining (blue), Syp-YFP (green), and varicosities along the axon (red) in an EP16 brain. White and yellow arrowheads indicate AIS and off-target varicosities, respectively. Filled and empty arrowheads show Syp-YFP+ and Syp-YFP- varicosities in ChC axons, respectively. Scale bar, 5 μm. D, The average percentage of Syp-YFP-containing AIS and off-target varicosities in EP16 ChCs. The percentage of Syp-YFP containing AIS varicosities is significantly higher than that of Syp-YFP containing off-target varicosities (n = 6 ChCs; ≥3 brains; Student’s t test: ***p < 0.001)l. Data are presented as mean ± SEM. E, The average percentage of VGAT-containing AIS and off-target varicosities in P28 ChCs. The percentage of VGAT-containing AIS varicosities is significantly higher than that of VGAT-containing off-target varicosities (n = 7 ChCs for each; ≥3 brains per condition; Student’s t test: ***p < 0.001)°. Data are presented as mean ± SEM.

    AIS but not off-target varicosities predominantly contain presynaptic markers. A, Immunohistochemical analysis of VGAT localization in ChC varicosities in Nkx2.1-CreER;LSL-tGFP mice. Confocal images of a single optical section showing varicosities (green), VGAT (red), and AISs visualized by AnkG immunostaining (blue) in a P16 brain. White and yellow arrowheads indicate AIS and off-target varicosities, respectively. Filled and empty arrowheads show VGAT+ and VGAT- varicosities in ChC axons, respectively. Scale bar, 5 μm. B, The average percentage of VGAT-containing AIS and off-target varicosities in P16 ChCs...

    Read More

    Could Removal of Aging Cells extend human life?

    June 9, 2017, Categories  Health/Medical, Biology/Biotechnology

    Clearance of SnCs by GCV reduces the development of post-traumatic OA. Credit: UNIST

    Clearance of SnCs by GCV reduces the development of post-traumatic OA.
    Credit: UNIST

    A recent study, led by an international team confirms that targeted removal of senescent cells (SnCs), accumulated in many vertebrate tissues as we age, contribute significantly in delaying the onset of age-related pathologies. This breakthrough research has been led by Dr. Chaekyu Kim of the Johns Hopkins University School of Medicine, now at UNIST, and Dr. Ok Hee Jeon of the Johns Hopkins University School of Medicine in collaborations with the Mayo Clinic College of Medicine, the Buck Institute for Research on Aging, the University Medical Center Groningen, Unity Biotechnology, Inc., and the University of California, Berkeley.

    In the study, the team presented a novel pharmacologic candidate that alleviat...

    Read More

    Tackling Infectious Disease, one Protein at a time

    June 9, 2017, Categories  Health/Medical, Biology/Biotechnology

    1.The structure of a protein named thioredoxin. Garry Buchko and colleagues used NMR to solve the structure of the protein, which is found in an infection often conveyed by ticks. Credit: SSGCID 2. A protein in the pathogen that causes cryptosporidiosis. The microbe can cause mild to severe diarrhea in people who accidentally swallow a mouthful of contaminated water. Credit: SSGCID 3.Buchko and colleagues solved this structure of a protein found in the organism that causes malaria. Credit: SSGCID 4. A protein in the microbe that causes melioidosis, which occurs most often in people who live in tropical climates. Infection often starts in the lungs when contaminated dust or soil is inhaled. Credit: SSGCID 5. A protein in the micro-organism that causes giardiasis, which translates to nausea, abdominal pain, fatigue and other symptoms in hundreds of millions of people worldwide each year. Credit: SSGCID

    1.The structure of a protein thioredoxin. Garry Buchko and colleagues used NMR to solve the structure of the protein, which is found in an infection often conveyed by ticks. Credit: SSGCID
    2. A protein in the pathogen that causes cryptosporidiosis. The microbe can cause mild to severe diarrhea in people who accidentally swallow a mouthful of contaminated water. Credit: SSGCID
    3.Buchko and colleagues solved this structure of a protein found in the organism that causes malaria. Credit: SSGCID
    4. A protein in the microbe that causes melioidosis, which occurs most often in people who live in tropical climates. Infection often starts in the lungs when contaminated dust or soil is inhaled. Credit: SSGCID
    5...

    Read More

    Red Onions pack a Cancer-fighting punch, study reveals

    June 8, 2017, Categories  Health/Medical, Biology/Biotechnology

    Abdulmonem I. Murayyan, Cynthya M. Manohar, Gordon Hayward, Suresh Neethirajan. Antiproliferative activity of Ontario grown onions against colorectal adenocarcinoma cells. Food Research International, 2017; 96: 12 DOI: 10.1016/j.foodres.2017.03.017

    Abdulmonem I. Murayyan, Cynthya M. Manohar, Gordon Hayward, Suresh Neethirajan. Antiproliferative activity of Ontario grown onions against colorectal adenocarcinoma cells. Food Research International, 2017; 96: 12 DOI: 10.1016/j.foodres.2017.03.017

    Not all onions are created equal. Engineering professor Suresh Neethirajan and PhD student Abdulmonem Murayyan tested 5 onion types grown in Ontario and discovered the Ruby Ring onion variety came out on top. Onions as a superfood are still not well known. But they contain one of the highest concentrations of quercetin, a type of flavonoid, and Ontario onions boasts particularly high levels of the compound compared to some parts of the world.

    The Guelph study revealed that the red onion not only has high levels of quercetin, but also high amount...

    Read More