aging tagged posts

Effects of pNaKtide on adipocyte phenotype, senescence, and apoptosis in C57Bl6 aging mice. (A) H&E staining in visceral adipose tissue. Images taken with 40X objective lens; scale bar represents 100 µm. Arrows mark “crown like structures” indicative of inflammation. (B) Quantitative analysis of adipocytes area in visceral adipose tissue. (C) Representative images of TUNEL assay with quantification in C57B16 aging mice. Images taken with 40X objective lens; scale bar represents 25 µm. (D–F) qRT-PCR analysis of ApoJ, p21 and PPARγ in C57Bl6 aging mice with GAPDH as a loading control. Y, young; Y + P, young + pNaKtide; OB, old baseline; O, old; O + P, old + pNaKtide; O + WD, old + western diet; O + WD + P, old + western diet + pNaKtide. N = 8/group, *p < 0.05, **p < 0...

The structure of human telomerase reverse transcriptase (hTERT). Human TERT contains four key domains: the telomerase essential N-terminal (TEN) domain; the TERT RNA binding (TRB) domain; the reverse transcriptase (RT) domain and the C-terminal extension (CTE) domain.

With disease, when you decrease autophagy (a garbage disposal-like process where cells “eat” debris they produce) the disease process is exacerbated and when you increase it you get the opposite effect. Aggregation of polyglutamine expansion protein is a hallmark of Huntington’s disease and other neurodegenerative diseases. The picture shows that there are more aggregates of green fluorescence protein-labelled polyglutamine expansion protein in autophagy deficient worms (bottom) compared to normal worms (top).