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    controlling autophagy tagged posts

    KLF-mediated lifespan extension is dependent on autophagy. Klf-3 transcript levels in wild-type animals subjected to 2 days of chronic dietary restriction (sDR, OP50 diluted to 108 cfu/ml) (a) or inhibition of TOR signaling by RNAi against let-363 (b) starting from day 1 of adulthood. All lines were raised and maintained at 20 °C. *P-value < 0.05 by Student’s T-test, N = 3 biological replicates. c qPCR analysis of a panel of autophagy-related genes in day 1 klf-3 o/e and loss of function of both klf-3 and klf-1 animals compared to wild type. Double loss of function of klf-3 and klf-1 was performed as described previously utilizing the klf-3(ok175) mutant and simultaneous RNAi feeding targeting klf-1. All lines were raised and maintained at 20 °C. *P-value < 0.05 by Student’s T-test, N = 3 biological replicates (d, representative picture). e Autophagy in klf-3 o/e animals as determined by numbers of GFP::LGG-1 punctae in seam cells (red arrow denotes GFP-positive puncta) with knockdown of bec-1 in both wild-type and klf-3 o/e animals. *P-value < 0.05, # P-value ≤ 0.1 after one-way analysis of variance followed by the Tukey’s post hoc test. N = 10–20 animals counted. (f, wild-type representative image, g, klf-3 o/e representative image) Electron microscopy images of klf-3 o/e and wild-type animals in animals aged 9 days. Arrowheads indicate sizeable (≥500 nm) autolysosomes as recognized by single-membrane limited vacuolar structures with visible mixed cytoplasmic contents. Full images reproduced in Supplementary Fig. 11, with additional images. h Lifespan analysis of wild-type and klf-3 o/e animals fed RNAi bacteria targeting bec-1 from day 1 of adulthood. All lines were raised and maintained at 20 °C. P-value < 0.05 by Mantel–Cox log-rank tests. See also Supplementary Table 6 for details of lifespan analyses and replicate experiments. All error bars represent standard error of the mean (SEM)

    KLF-mediated lifespan extension is dependent on autophagy. Klf-3 transcript levels in wild-type animals subjected to 2 days of chronic dietary restriction (sDR, OP50 diluted to 108 cfu/ml) (a) or inhibition of TOR signaling by RNAi against let-363 (b) starting from day 1 of adulthood. All lines were raised and maintained at 20 °C. *P-value < 0.05 by Student’s T-test, N = 3 biological replicates. c qPCR analysis of a panel of autophagy-related genes in day 1 klf-3 o/e and loss of function of both klf-3 and klf-1 animals compared to wild type. Double loss of function of klf-3 and klf-1 was performed as described previously utilizing the klf-3(ok175) mutant and simultaneous RNAi feeding targeting klf-1. All lines were raised and maintained at 20 °C. *P-value < 0...

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