CRISPR-Cas9 gene editing system tagged posts

Highlights •Bacteriophage anti-CRISPR proteins inactivate Listeria monocytogenes CRISPR-Cas9 •Half of L. monocytogenes isolates possess inhibited CRISPR-Cas9 systems •AcrIIA2 and AcrIIA4 prevent target binding by dCas9 in bacteria •AcrIIA2 and AcrIIA4 inhibit Cas9-mediated gene editing in human cells

UCSF researchers have discovered a way to switch off the CRISPR-Cas9 gene-editing system using newly identified anti-CRISPR proteins that are produced by bacterial viruses. The technique has the potential to improve the safety and accuracy of CRISPR applications both in the clinic and for basic research...