destruction of mitotic spindles tagged posts

Aberrant spindles and impaired spindle poles in human cancer cells treated with the phenanthridine PJ34. (A) Small aberrant spindles were identified by confocal microscopy in randomly scanned human breast cancer MDA-MB-231 cells incubated with PJ34 (20 μM, 27 h). Spindles were immunolabeled for HSET/kifC1 (green) bound to microtubules. Similarly immunolabeled spindles are not impaired in PJ34 treated normal human breast epithelial cells MCF10A. Bars indicate the spindle length measured by the scale bar (n = 20; 3 different experiments), 95% of the spindles in MDA-MB-231 cells were shorter, 60 ± 5% in size. (B) Upper panel: Impaired spindle poles were identified in randomly scanned fixed MDA-MB-231 cells treated with PJ34 (20 μM, 27 h)...