Recent Comments

    hnRNP A1 tagged posts

    Protein-RNA Structure hints at how Viruses Commandeer Human Proteins

    November 11, 2015, Categories  Health/Medical, Biology/Biotechnology

    Highlights • The first crystal structure of the UP1 domain of hnRNP A1 bound to RNA is determined. • The UP1–RNA structure reveals that only the RRM1 sub-domain and inter-RRM linker of UP1 interact with the RNA via a nucleobase pocket; RRM2 does not contact the RNA. • A structural model scored by small-angle X-ray scattering reveals UP1 binds the HIV ESS3 stem loop as a 1:1 complex using its RRM1 domain and inter-RRM linker only. • Structure-guided mutagenesis and molecular dynamics simulations show the RRM1 and RRM2 sub-domains synergize to fold the inter-RRM linker in a conformation that determines specific and high-affinity RNA interactions.

    Highlights • The first crystal structure of the UP1 domain of hnRNP A1 bound to RNA is determined. • The UP1–RNA structure reveals that only the RRM1 sub-domain and inter-RRM linker of UP1 interact with the RNA via a nucleobase pocket; RRM2 does not contact the RNA. • A structural model scored by small-angle X-ray scattering reveals UP1 binds the HIV ESS3 stem loop as a 1:1 complex using its RRM1 domain and inter-RRM linker only. • Structure-guided mutagenesis and molecular dynamics simulations show the RRM1 and RRM2 sub-domains synergize to fold the inter-RRM linker in a conformation that determines specific and high-affinity RNA interactions.

    Researchers have produced the first image of an important human protein as it binds with ribonucleic acid (RNA), a discovery that could o...

    Read More