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    Nasopharyngeal Protein Biomarkers of Acute Respiratory Virus Infection tagged posts

    Proteins Biomarkers in your Runny Nose could Verify Viral infection from Cold or Flu

    February 22, 2017, Categories  Health/Medical, Biology/Biotechnology

    Study design and experimental workflow. A two-phased strategy was employed to identify and characterize candidate protein biomarkers of ARV infection from NPL samples collected from participants in four experimental ARV challenge cohorts. For phase 1 discovery analysis, four NPL pools were prepared from H3N2 #1 cohort and analyzed using unbiased 2D-LC-MS/MS. The numbers of subject (N) with samples included in each pool are shown (Uninf = uninfected individuals; Inf = infected individuals; BL = baseline; T = time of maximal symptoms). For phase 2, the original and three additional independent challenge cohorts were assayed by targeted MRM. Quantitative peptide expression data from 80 individuals and 156 total samples were used in the derivation of an NPL ARV classifier, and classification performance was assessed in independent challenge cohorts using LOOCV.

    Study design and experimental workflow. A two-phased strategy was employed to identify and characterize candidate protein biomarkers of ARV infection from NPL samples collected from participants in four experimental ARV challenge cohorts. For phase 1 discovery analysis, four NPL pools were prepared from H3N2 #1 cohort and analyzed using unbiased 2D-LC-MS/MS. The numbers of subject (N) with samples included in each pool are shown (Uninf = uninfected individuals; Inf = infected individuals; BL = baseline; T = time of maximal symptoms). For phase 2, the original and three additional independent challenge cohorts were assayed by targeted MRM...

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