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    Skin Cells found at Root of Balding, Gray Hair

    May 7, 2017, Categories  Health/Medical, Biology/Biotechnology

    Mice lacking SCF in Krox20 lineage cells exhibit progressive hair graying. (A) A representative Scfflox/gfp; Krox20Cre mouse (top) displaying hair graying along with aging as compared with a littermate Scfflox/gfp control (bottom). n = 20. (B) Pelage hairs plucked from P20 Scfflox/gfp; Krox20Cre mice showed distinct pigment levels in distal and proximal ends; newly developed hairs in the proximal side (arrowheads) were absent of pigment. (C) Fontana-Masson staining confirmed the absence of melanin in the newly synthesized hairs (arrowheads) in P32 Scfflox/gfp; Krox20Cre mice. (D) HF section revealing normal hair pigmentation in Scfflox/gfp; Krox20Cre mice at P9 followed by a rapid loss of pigmentation beginning from the hair matrix (arrowheads) at P11 and P13. (E) Transverse HF section revealing hypopigmented hair shafts (arrowheads) in Scfflox/gfp; Krox20Cre mice at P11 and P13. (F) Quantification and statistical analysis of E showing a significant reduction of melanin density in Scfflox/gfp; Krox20Cre graying hair shafts. Melanin density was analyzed by ImageJ. Data are mean ± SEM from 100 randomly selected HFs in each group. (∗∗∗∗) P < 0.0001. (G) Gray Scfflox/gfp; Krox20Cre mice were depilated to stimulate hair regeneration; new hair shafts were completely absent of pigment. n = 3. Nuclei were stained with nuclear Fast Red in C–E. Bar, 100 µm.

    Mice lacking SCF in Krox20 lineage cells exhibit progressive hair graying. (A) A representative Scfflox/gfp; Krox20Cre mouse (top)
    displaying hair graying along with aging as compared with a littermate Scfflox/gfp control (bottom). n = 20. (B) Pelage hairs plucked from P20
    Scfflox/gfp; Krox20Cre mice showed distinct pigment levels in distal and proximal ends; newly developed hairs in the proximal side (arrowheads)
    were absent of pigment. (C) Fontana-Masson staining confirmed the absence of melanin in the newly synthesized hairs (arrowheads)
    in P32 Scfflox/gfp; Krox20Cre mice. (D) HF section revealing normal hair pigmentation in Scfflox/gfp; Krox20Cre mice at P9
    followed by a rapid loss of pigmentation beginning from the hair matrix (arrowheads) at P11 and P13...

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