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    Category Biology/Biotechnology

    KLF-mediated lifespan extension is dependent on autophagy. Klf-3 transcript levels in wild-type animals subjected to 2 days of chronic dietary restriction (sDR, OP50 diluted to 108 cfu/ml) (a) or inhibition of TOR signaling by RNAi against let-363 (b) starting from day 1 of adulthood. All lines were raised and maintained at 20 °C. *P-value < 0.05 by Student’s T-test, N = 3 biological replicates. c qPCR analysis of a panel of autophagy-related genes in day 1 klf-3 o/e and loss of function of both klf-3 and klf-1 animals compared to wild type. Double loss of function of klf-3 and klf-1 was performed as described previously utilizing the klf-3(ok175) mutant and simultaneous RNAi feeding targeting klf-1. All lines were raised and maintained at 20 °C. *P-value < 0.05 by Student’s T-test, N = 3 biological replicates (d, representative picture). e Autophagy in klf-3 o/e animals as determined by numbers of GFP::LGG-1 punctae in seam cells (red arrow denotes GFP-positive puncta) with knockdown of bec-1 in both wild-type and klf-3 o/e animals. *P-value < 0.05, # P-value ≤ 0.1 after one-way analysis of variance followed by the Tukey’s post hoc test. N = 10–20 animals counted. (f, wild-type representative image, g, klf-3 o/e representative image) Electron microscopy images of klf-3 o/e and wild-type animals in animals aged 9 days. Arrowheads indicate sizeable (≥500 nm) autolysosomes as recognized by single-membrane limited vacuolar structures with visible mixed cytoplasmic contents. Full images reproduced in Supplementary Fig. 11, with additional images. h Lifespan analysis of wild-type and klf-3 o/e animals fed RNAi bacteria targeting bec-1 from day 1 of adulthood. All lines were raised and maintained at 20 °C. P-value < 0.05 by Mantel–Cox log-rank tests. See also Supplementary Table 6 for details of lifespan analyses and replicate experiments. All error bars represent standard error of the mean (SEM)

    KLF-mediated lifespan extension is dependent on autophagy. Klf-3 transcript levels in wild-type animals subjected to 2 days of chronic dietary restriction (sDR, OP50 diluted to 108 cfu/ml) (a) or inhibition of TOR signaling by RNAi against let-363 (b) starting from day 1 of adulthood. All lines were raised and maintained at 20 °C. *P-value < 0.05 by Student’s T-test, N = 3 biological replicates. c qPCR analysis of a panel of autophagy-related genes in day 1 klf-3 o/e and loss of function of both klf-3 and klf-1 animals compared to wild type. Double loss of function of klf-3 and klf-1 was performed as described previously utilizing the klf-3(ok175) mutant and simultaneous RNAi feeding targeting klf-1. All lines were raised and maintained at 20 °C. *P-value < 0...

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    Like it or not: Broccoli may be Good for the Gut

    October 13, 2017, Categories  Health/Medical, Biology/Biotechnology

    Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner. Journal of Functional Foods, 2017; 37: 685 DOI: 10.1016/j.jff.2017.08.038

    Dietary broccoli impacts microbial community structure and attenuates chemically induced colitis in mice in an Ah receptor dependent manner. Journal of Functional Foods, 2017; 37: 685 DOI: 10.1016/j.jff.2017.08.038

    For the broccoli haters of the world, researchers may have more bad news: the vegetable may also help promote a healthy gut. In a study, when mice ate broccoli with their regular diet, they were better able to tolerate digestive issues similar to symptoms of leaky gut and colitis than mice that were not placed on a broccoli-supplemented diet, according to Gary Perdew, the John T. and Paige S. Smith Professor in Agricultural Sciences, Penn State. He added that other vegetables, like brussels sprouts and cauliflower, may also have similar gut health properties.

    “There are a lot of...

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    Future Smartwatches could Sense Hand Movement using Ultrasound imaging

    October 12, 2017, Categories  Physics, Technology/Electronics, Biology/Biotechnology

    This is ultrasonic imaging of the forearm. Credit: Copyright BIG, University of Bristle 65

    This is ultrasonic imaging of the forearm.
    I8

    New research has shown future wearable devices, such as smartwatches, could use ultrasound imaging to sense hand gestures. Computers are growing in number and wearable computers, such as smartwatches, are gaining popularity. Devices around the home, such as WiFi light bulbs and smart thermostats, are also on the increase. However, current technology limits the capability to interact with these devices. Hand gestures have been suggested as an intuitive and easy way of interacting with and controlling smart devices in different surroundings. For instance, a gesture could be used to dim the lights in the living room, or to open or close a window...

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    Breast Cancer linked to Bacterial Imbalances

    October 11, 2017, Categories  Health/Medical, Biology/Biotechnology

    Methalobacterkum

    Study compares cterial composition in healthy vs. cancerous breast tissue. The Cleveland team has discovered for the first time that healthy breast tissue contains more of the bacterial species Methylobacterium, a finding which could offer a new perspective in the battle against breast cancer. Researchers have long suspected that a “microbiome” exists within breast tissue and plays a role in breast cancer but it has not yet been characterized. The research team has taken the first step toward understanding the composition of the bacteria in breast cancer by uncovering distinct microbial differences in healthy and cancerous breast tissue.

    “To my knowledge, this is the first study to examine both breast tissue and distant sites of the body for bacterial differences in breast...

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