Category Health/Medical

Nanosecond Pulsed Electric fields activate Immune cells

figure3
Analysis of extracellular DNA by agarose gel electrophoresis. (A) Schematic representation of MNase treatment for liberation of extruded DNA from cells. MNase digests extracellular chromosomal DNA at its linker regions among nucleosomes, leading to the liberation of extracellular chromosomal DNA from their originating cells. Liberated DNA was separated from cells by brief centrifugation and in turn subjected to either DNA purification followed by agarose gel electrophoresis (B) or direct fluorometric measurement with a SYTOX Green dye (Fig. 4). (B) Analysis of MNase-treated extracellular DNA by agarose gel electrophoresis. Differentiated (D) and undifferentiated cells (U) were exposed to the indicated shot numbers of 20 kV/cm nsPEFs...
Read More

Direct Detection of Circulating Tumor cells in Blood Samples

Enhanced Glow: Direct detection of circulating tumor cells in blood samples
Enhanced Glow: Direct detection of circulating tumor cells in blood samples

Tumor cells circulating in blood are markers for the early detection and prognosis of cancer. However, detection of these cells is challenging because of their scarcity. In the journal Angewandte Chemie, scientists have now introduced an ultrasensitive method for the direct detection of circulating tumor cells in blood samples. It is based on the amplified, time-resolved fluorescence measurement of luminescent lanthanide ions released from nanoparticles that bind specifically to tumor cells.

Conventional techniques for the detection of circulating tumor cells require complicated enrichment before detection because a sample of 10 million blood cells only contains about one tumor cell...

Read More

Key Proteins for the Repair of Nerve Fibers Identified

ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNS

Scientists at the German Center for Neurodegenerative Diseases (DZNE) have identified a group of proteins that help to regenerate damaged nerve cells. Their findings are reported in the journal Neuron.

It is commonly accepted that neurons of the central nervous system shut down their ability to grow when they no longer need it; this occurs normally after they have found their target cells and established synapses. However, recent findings show that old nerve cells have the potential to regrow and to repair damage similar to young neurons...

Read More

Novel Nano-Vaccine for Melanoma

Melanoma in skin biopsy with H&E stain — this case may represent superficial spreading melanoma. Credit: Wikipedia/CC BY-SA 3.0

Injection of Nanoparticle has proven effective in Mouse Models, researchers say. Researchers at Tel Aviv University have developed a novel nano-vaccine for melanoma, the most aggressive type of skin cancer. Their innovative approach has so far proven effective in preventing the development of melanoma in mouse models and in treating primary tumors and metastases that result from melanoma.

The focus of the research is on a nanoparticle that serves as the basis for the new vaccine. The study was led by Prof...

Read More